Product Name
CHRNA2, Polyclonal Antibody
Full Product Name
CHRNA2 Polyclonal Antibody
Product Gene Name
anti-CHRNA2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15822
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human CHRNA2
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-CHRNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-CHRNA2 antibody
Polyclonal
Applications Tested/Suitable for anti-CHRNA2 antibody
Western Blot (WB)
Application Notes for anti-CHRNA2 antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-CHRNA2 antibody
Western blot analysis of extracts of rat skeletal muscle, using CHRNA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
NCBI/Uniprot data below describe general gene information for CHRNA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000733.2
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NCBI GenBank Nucleotide #
NM_000742.3
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UniProt Primary Accession #
Q15822
[Other Products]
UniProt Secondary Accession #
Q9HAQ3; A8KAX3; B4DK19; J3KMY9[Other Products]
UniProt Related Accession #
Q15822[Other Products]
Molecular Weight
Calculated MW: 60kDa
Molecular Weight: 529
NCBI Official Full Name
neuronal acetylcholine receptor subunit alpha-2 isoform 1
NCBI Official Synonym Full Names
cholinergic receptor nicotinic alpha 2 subunit
NCBI Official Symbol
CHRNA2 [Similar Products]
NCBI Protein Information
neuronal acetylcholine receptor subunit alpha-2
UniProt Protein Name
Neuronal acetylcholine receptor subunit alpha-2
Protein Family
Neuronal acetylcholine receptor
UniProt Gene Name
CHRNA2 [Similar Products]
UniProt Entry Name
ACHA2_HUMAN
NCBI Summary for CHRNA2
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]
UniProt Comments for CHRNA2
nAChRA2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4). ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 2/CHRNA2 sub-subfamily.
Protein type: Channel, ligand-gated; Channel, cation; Receptor, misc.; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: cell junction; integral to membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic membrane
Molecular Function: acetylcholine binding; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity
Biological Process: ion transport; neuromuscular synaptic transmission; protein heterooligomerization; response to nicotine; signal transduction; synaptic transmission; synaptic transmission, cholinergic
Disease: Epilepsy, Nocturnal Frontal Lobe, 4
Research Articles on CHRNA2
1. T; p. Arg376Trp) in subjects with benign familial infantile seizures">a heterozygous single-nucleotide substitution in CHRNA2 gene (c.1126 C>T; p. Arg376Trp) in subjects with benign familial infantile seizures
Precautions
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