Hamartin/TSC1, Polyclonal Antibody

TSC1; hamartin; tuberous sclerosis 1; HGNC: 12362; KIAA0243; LAM; MGC86987; TSC; tuberous sclerosis 1 protein; tumor suppressor; TSC1 antibody; hamartin antibody; tuberous sclerosis 1 antibody; HGNC: 12362 antibody; KIAA0243 antibody; LAM antibody; MGC86987 antibody; TSC antibody; tuberous sclerosis 1 protein antibody; tumor suppressor antibody; Hamartin; Hamartin / TSC1 (isoform 1)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-TSC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet.2000 Jul 22;9(12): 1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx.120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.

(0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Human TSC1 (RC213332) and probed (mock transfection in first lane), tested by Origene.

(3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

124,015 Da

tuberous sclerosis 1

hamartin

Hamartin

KIAA0243; TSC  [Similar Products]

TSC1_HUMAN

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: actin filament; cell cortex; cytoplasm; cytosol; growth cone; lamellipodium; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; TSC1-TSC2 complex

Molecular Function: chaperone binding; GTPase activating protein binding; GTPase regulator activity; protein binding; protein N-terminus binding

Biological Process: adaptive immune response; ***** locomotory behavior; cardiac muscle cell differentiation; cell cycle arrest; cell projection organization and biogenesis; cell-matrix adhesion; cerebral cortex development; glucose import; hippocampus development; kidney development; memory T cell differentiation; myelination; negative regulation of cell proliferation; negative regulation of cell size; negative regulation of insulin receptor signaling pathway; negative regulation of macroautophagy; negative regulation of TOR signaling pathway; negative regulation of translation; neural tube closure; positive regulation of focal adhesion formation; potassium ion transport; protein heterooligomerization; protein stabilization; regulation of cell cycle; regulation of cell-matrix adhesion; regulation of phosphoprotein phosphatase activity; regulation of protein kinase activity; regulation of stress fiber formation; regulation of translation; response to insulin stimulus; rRNA export from nucleus; synapse organization and biogenesis

Disease: Focal Cortical Dysplasia Of Taylor; Lymphangioleiomyomatosis; Tuberous Sclerosis 1

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