Neuronal acetylcholine receptor subunit alpha-2, ELISA Kit

Neuronal acetylcholine receptor subunit alpha-2; CHRNA2

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of CHRNA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2883803 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuronal acetylcholine receptor subunit alpha-2 (CHRNA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHRNA2. The ELISA analytical biochemical technique of the MBS2883803 kit is based on CHRNA2 antibody-CHRNA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRNA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRNA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

58,140 Da

cholinergic receptor nicotinic alpha 2 subunit

neuronal acetylcholine receptor subunit alpha-2

Neuronal acetylcholine receptor subunit alpha-2

ACHA2_HUMAN

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]

nAChRA2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4). ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 2/CHRNA2 sub-subfamily.

Protein type: Membrane protein, integral; Receptor, misc.; Channel, ligand-gated; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: cell junction; integral to membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity

Biological Process: ion transport; neuromuscular synaptic transmission; protein heterooligomerization; response to nicotine; signal transduction; synaptic transmission; synaptic transmission, cholinergic

Disease: Epilepsy, Nocturnal Frontal Lobe, 4

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