iNOS, Polyclonal Antibody

Nitric oxide synthase, inducible; nitric oxide synthase 2, inducible; HEP NOS antibody; HEP-NOS antibody; Hepatocyte NOS antibody; HEPNOS antibody; inducible antibody; Inducible nitric oxide synthase antibody; Inducible NO synthase antibody; Inducible NOS antibody; INOS antibody; Inosl antibody; MAC NOS antibody; Macrophage NOS antibody; Nitric oxide synthase 2 inducible antibody; Nitric oxide synthase 2 inducible macrophage antibody; Nitric oxide synthase 2A(inducible hepatocytes) antibody; Nitric oxide synthase antibody; Nitric oxide synthase inducible antibody; NOS 2 antibody; NOS 2A antibody; NOS antibody; Nos II antibody; NOS type II antibody; Nos2 antibody; NOS2_HUMAN antibody; NOS2A antibody; Peptidyl-cysteine S-nitrosylase NOS2 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-NOS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Nitric oxide synthase, inducible(NOS2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide(NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS(iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS(NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. Diefenbach et al.(1999) studied the relationship of IL12 and nitric oxide synthase-2(NOS2) to innate immunity to the parasite Leishmania in mice. And conclude that NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity. From studies in Tanzania and Kenya, Hobbs et al.(2002) identified a novel single-nucleotide polymorphism, -1173C-T(163730.0001), in the NOS2 promoter that was significantly associated with protection from symptomatic malaria and severe malarial anemia.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

126,749 Da

nitric oxide synthase 2, inducible

nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)

Nitric oxide synthase, inducible

NOS2A; HEP-NOS; Inducible NOS; iNOS  [Similar Products]

NOS2_HUMAN

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; cytosol; nucleus

Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; receptor binding

Biological Process: circadian rhythm; interaction with host; superoxide metabolic process; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of vasodilation; defense response to Gram-negative bacterium; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of blood pressure; nitric oxide biosynthetic process; blood coagulation; negative regulation of protein catabolic process; inflammatory response; nitric oxide mediated signal transduction; regulation of insulin secretion

Disease: Hypertension, Essential; Malaria, Susceptibility To

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