ARFGEF2, Antibody

BIG2; brefeldin A-inhibited GEP 2; brefeldin A-inhibited guanine nucleotide-exchange protein 2

For Research Use Only. Not for use in diagnostic procedures.

ARFGEF2 Antibody detects endogenous levels of total ARFGEF2

Immunogen affinity purified

In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C. Stable for 12 months from date of receipt.

Small volumes of anti-ARFGEF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: 1:500~1:1000
IF/ICC: 1:100-1:500

Western blot analysis of extracts from A549 cells, using ARFGEF2 antibody.

210 KD

ADP ribosylation factor guanine nucleotide exchange factor 2

brefeldin A-inhibited guanine nucleotide-exchange protein 2

Brefeldin A-inhibited guanine nucleotide-exchange protein 2

ARFGEP2; BIG2; Brefeldin A-inhibited GEP 2  [Similar Products]

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008]

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.

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