CHRNA2, Polyclonal Antibody

Neuronal acetylcholine receptor subunit alpha-2; CHRNA2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CHRNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-69 amino acids from the N-terminal region of human CHRNA2.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CHRNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Nicotinic acetylcholine receptors (nAChRs) are
ligand-gated ion channels formed by a pentameric arrangement of
alpha and beta subunits to create distinct muscle and neuronal
receptors. Neuronal receptors are found throughout the peripheral
and central nervous system where they are involved in fast synaptic
transmission. This gene encodes an alpha subunit that is widely
expressed in the brain. The proposed structure for nAChR subunits
is a conserved N-terminal extracellular domain followed by three
conserved transmembrane domains, a variable cytoplasmic loop, a
fourth conserved transmembrane domain, and a short C-terminal
extracellular region. Mutations in this gene cause autosomal
dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide
polymorphisms (SNPs) in this gene have been associated with
nicotine dependence.

Neuroscience

Western Blot (WB), ELISA (EIA)

WB~~1:1000

CHRNA2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CHRNA2 antibody detected the CHRNA2 protein (arrow).

59765

cholinergic receptor, nicotinic, alpha 2 (neuronal)

neuronal acetylcholine receptor subunit alpha-2

Neuronal acetylcholine receptor subunit alpha-2

ACHA2_HUMAN

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]

nAChRA2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4). ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 2/CHRNA2 sub-subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated; Channel, cation; Receptor, misc.

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: acetylcholine receptor activity; drug binding; nicotinic acetylcholine-activated cation-selective channel activity; acetylcholine binding

Biological Process: synaptic transmission; protein heterooligomerization; ion transport; signal transduction; cation transport

Disease: Epilepsy, Nocturnal Frontal Lobe, 4

Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Rigbi, A., et al. Pharmacogenomics J. (2010) In press :
Hoda, J.C., et al. FEBS Lett. 583(10):1599-1604(2009)
Philibert, R.A., et al. Nicotine Tob. Res. 11(3):286-292(2009)

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