Nicotinic Receptor, beta 2, aa493-502, Polyclonal Antibody

Anti -Nicotinic Receptor, beta 2, CT, aa493-502

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 8; NC_000008.10 (27317278..27336813, complement). Location: 8p21

Polyclonal

IgG

Rabbit

Recognizes Human Nicontinic beta 2, (carboxyl terminal) Receptor (a.a. 493-502). Crossreactivity: Nicotinic beta 2 receptor (493-502) 100%; Nicotine beta 2 receptor ~90%; Nicotinic alpha 3 receptor 0%; Nicotinic alpha 4 receptor 0%; Nicotinic alpha 5 receptor 0%; Nicotinic alpha 7 receptor 0%; Nicotinic beta 3 receptor 0%; Nicotinic beta 4 receptor 0%.

Serum
Serum

Supplied as a lyophilized powder. Reconstitute with 0.1ml 40-50% glycerol, PBS, 10mg/ml BSA.

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile40-50% glycerol, PBS, 10mg/ml BSA, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-CHRNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Antibodies; Abs to Receptors, Cholinergic

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)

Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Dilution: Immunocytochemistry: 1:200. Stains fixed cells known to express the nicotinic beta 2 receptor using ABC techniques.
Western Blot: 1:400. Using tissue homogenates to yield a single band at 60kD.

59,765 Da[Similar Products]

cholinergic receptor, nicotinic, alpha 2 (neuronal)

neuronal acetylcholine receptor subunit alpha-2; acetylcholine receptor, nicotinic, alpha 2 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal)

Neuronal acetylcholine receptor subunit alpha-2

ACHA2_HUMAN

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]

Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure: Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Involvement in disease: Epilepsy, nocturnal frontal lobe, 4 (ENFL4) [MIM:610353]: An autosomal dominant focal epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily. [View classification]

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