Product Name
CLCN7, siRNA
Full Product Name
CLCN7 siRNA (Human)
Product Synonym Names
H(+)/Cl(-) exchange transporter 7; Chloride channel 7 alpha subunit; Chloride channel protein 7; ClC-7
Product Gene Name
CLCN7 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51798
Specificity
CLCN7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CLCN7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CLCN7 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CLCN7 sirna
siRNA to inhibit CLCN7 expression using RNA interference
Applications Tested/Suitable for CLCN7 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CLCN7. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107803.1
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NCBI GenBank Nucleotide #
NM_001114331.2
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UniProt Primary Accession #
P51798
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UniProt Secondary Accession #
Q9NYX5; A6NEJ7; A8K5T9; A8K7X1; B3KPN3; E9PDB9[Other Products]
UniProt Related Accession #
P51798[Other Products]
Molecular Weight
86,026 Da
NCBI Official Full Name
H(+)/Cl(-) exchange transporter 7 isoform b
NCBI Official Synonym Full Names
chloride channel, voltage-sensitive 7
NCBI Official Symbol
CLCN7 [Similar Products]
NCBI Official Synonym Symbols
CLC7; CLC-7; OPTA2; OPTB4; PPP1R63
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NCBI Protein Information
H(+)/Cl(-) exchange transporter 7
UniProt Protein Name
H(+)/Cl(-) exchange transporter 7
UniProt Synonym Protein Names
Chloride channel 7 alpha subunit; Chloride channel protein 7; ClC-7
Protein Family
H(+)/Cl(-) exchange transporter
UniProt Gene Name
CLCN7 [Similar Products]
UniProt Synonym Gene Names
ClC-7 [Similar Products]
UniProt Entry Name
CLCN7_HUMAN
NCBI Summary for CLCN7
The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or *****hood. [provided by RefSeq, Jul 2008]
UniProt Comments for CLCN7
CLCN7: Slowly voltage-gated channel mediating the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the lysosome lumen. Defects in CLCN7 are the cause of osteopetrosis autosomal recessive type 4 (OPTB4); also known as infantile malignant osteopetrosis type 2. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or *****hood. Defects in CLCN7 are the cause of osteopetrosis autosomal dominant type 2 (OPTA2); also known as autosomal dominant Albers-Schonberg disease or marble disease autosomal dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or *****hood. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or *****hood. It is characterized by sclerosis, predominantly involving the spine, the pelvis and the skull base. Belongs to the chloride channel (TC 2.A.49) family. ClC-7/CLCN7 subfamily.
Protein type: Channel, chloride; Membrane protein, multi-pass; Transporter; Transporter, ion channel; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p13
Cellular Component: membrane; lysosomal membrane; integral to membrane; cytoplasmic vesicle
Molecular Function: chloride channel activity; voltage-gated chloride channel activity; ATP binding; antiporter activity
Biological Process: transport; transmembrane transport; response to pH
Disease: Osteopetrosis, Autosomal Dominant 2; Osteopetrosis, Autosomal Recessive 4
Research Articles on CLCN7
1. Results show that ClC-7 is strongly expressed in OUMS-27,a chondrocyte cell line and is responsible for Cl- current. Its downregulation during the hypoosmotic stress accompanying osteoarthritis progression is part of the complex etiology of the disease.
Precautions
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