Full Product Name
IFIH1 Antibody
Product Synonym Names
Interferon-induced with helicase C domain protein 1; Helicase with 2 CARD domains; Helicard; Melanoma differentiation-associated protein 5; MDA-5; RNA helicase-DEAD box protein 116; Murabutide down-regulated protein
Product Gene Name
anti-IFIH1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BYX4
Species Reactivity
Human, Mouse
Specificity
IFIH1 Antibody detects endogenous levels of total IFIH1 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human IFIH1.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-IFIH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IFIH1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-IFIH1 antibody
WB: 1:500~1:1000
ELISA: 1:40000
Western Blot (WB) of anti-IFIH1 antibody
Western blot analysis of lysates from Jurkat cells, using IFIH1 Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for IFIH1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_071451.2
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NCBI GenBank Nucleotide #
NM_022168.3
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UniProt Primary Accession #
Q9BYX4
[Other Products]
UniProt Secondary Accession #
Q2NKL6; Q6DC96; Q86X56; Q96MX8; Q9H3G6[Other Products]
NCBI Official Full Name
interferon-induced helicase C domain-containing protein 1
UniProt Protein Name
Interferon-induced helicase C domain-containing protein 1
UniProt Synonym Protein Names
Clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140 autoantigen; Helicase with 2 CARD domains; Helicard; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116
Protein Family
Interferon-induced helicase C domain-containing protein
UniProt Gene Name
IFIH1 [Similar Products]
UniProt Synonym Gene Names
MDA5; RH116; CADM-140 autoantigen; Helicard; MDA-5; RLR-2 [Similar Products]
UniProt Comments for IFIH1
Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
Precautions
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Disclaimer
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