4-trimethylaminobutyraldehyde dehydrogenase, ELISA Kit

ALDH4; ALDH7; ALDH9; E3; TMABADH; 4-trimethylaminobutyraldehyde dehydrogenase|R-aminobutyraldehyde dehydrogenase|aldehyde dehydrogenase (NAD+)|aldehyde dehydrogenase 9A1|aldehyde dehydrogenase E3 is

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat ALDH9A1. No significant cross-reactivity or interference between Rat ALDH9A1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ALDH9A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285817 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALDH9A1. The ELISA analytical biochemical technique of the MBS285817 kit is based on ALDH9A1 antibody-ALDH9A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH9A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH9A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ALDH9A1 in samples. An antibody specific for ALDH9A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALDH9A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH9A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH9A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

53,653 Da

aldehyde dehydrogenase 9 family, member A1

4-trimethylaminobutyraldehyde dehydrogenase

4-trimethylaminobutyraldehyde dehydrogenase

TMABADH  [Similar Products]

catalyzes the conversion of gamma-trimethylaminobutyraldehyde to gamma-butyrobetaine in carnitine biosynthesis [RGD, Feb 2006]

ALDH9A1: Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction. Belongs to the aldehyde dehydrogenase family.

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.2.1.3; EC 1.2.1.47; Lipid Metabolism - fatty acid; Lipid Metabolism - glycerolipid; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Secondary Metabolites Metabolism - limonene and pinene degradation

Chromosomal Location of Human Ortholog: 13q24

Cellular Component: cytosol; mitochondrion

Molecular Function: 3-chloroallyl aldehyde dehydrogenase activity; 4-trimethylammoniobutyraldehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; amine binding; aminobutyraldehyde dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; protein homodimerization activity

Biological Process: aldehyde metabolic process; carnitine biosynthetic process; carnitine metabolic process; kidney development; liver development; neurotransmitter biosynthetic process

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