Glypican 6, Monoclonal Antibody

Anti -Glypican 6 (Glypiated Proteoglycan 6)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 13; NC_000013.10 (93879078..95060274). Location: 13q32

Monoclonal

IgG1

7H114

Mouse

Recognizes human Glypican 6. Does not crossreact with recombinant human Glypican 2, 3 or 5.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-GPC6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Glypican 6 (glypiated proteoglycan 6) belongs to the family of membrane heparin sulfate proteoglycans that are linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. The glucosaminoglycans (GAGs) attachment sites of glypican family proteins are localized close to the cell membrane in the C-terminal region. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Mature human and mouse glypican share 98% amino acid sequence identity. Glypican 6 is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases.

Antibodies; Abs to Receptors

ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, Western Blot and Direct ELISA.
Dilution: Flow Cytometry: 2.5ug/10e6 cells using HepG2 human hepatocellular carcinoma cells.
Western Blot: 1ug/ml using human Glypican 6

62,736 Da[Similar Products]

glypican 6

glypican-6; OTTHUMP00000018552; glypican proteoglycan 6

Glypican-6

GPC6_HUMAN

The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. [provided by RefSeq]

GPC6: Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Enhances migration and invasion of cancer cells through WNT5A signaling. Defects in GPC6 are a cause of omodysplasia type 1 (OMOD1). OMOD1 is a rare autosomal recessive skeletal dysplasia characterized by severe congenital micromelia with shortening and distal tapering of the humeri and femora to give a club-like appearance. Typical facial features include a prominent forehead, frontal bossing, short nose with a depressed broad bridge, short columella, anteverted nostrils, long philtrum, and small chin. Point mutations leading to protein truncation, as well as larger genomic rearrangements resulting in exon deletions, have been found in family segregating omodysplasia type 1. All mutations identified in individuals affected by omodysplasia could lead to the absence of a functional protein, the mutant RNAs being suspected to be nonsense-mediated mRNA decay (NMD) targets. Even if the mRNA escapes NMD and is translated, all mutations are expected to disrupt the three-dimensional protein structure and often to abolish multiple highly conserved cysteine residues. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; cell migration; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Omodysplasia 1

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