Product Name
APCL / APC2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human APCL / APC2
Product Synonym Names
Anti-APCL / APC2 Antibody (Internal) IHC-plus; APC2; Adenomatosis polyposis coli 2; APC-like; APCL; Human APCL; APC2
Product Gene Name
anti-APCL antibody
[Similar Products]
Product Synonym Gene Name
APC2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95996
Species Reactivity
Human, Mouse
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (lot specific)
Immunogen Description
An 18 amino acid peptide near the center of human APC2.
Immunogen Type
Synthetic peptide
Immunogen
APCL / APC2 antibody was raised against an 18 amino acid peptide near the center of human APC2.
Antigen Modification
Internal
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-APCL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-APCL antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-APCL antibody
ELISA, ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: APC2 antibody can be used for detection of APC2 by Western blot at 1-2 ug/ml.
Immunohistochemistry (IHC) of anti-APCL antibody
Anti-APC2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry (IHC) of anti-APCL antibody
Anti-APC2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence (IF) of anti-APCL antibody
Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 ug/ml.
Immunocytochemistry (ICC) of anti-APCL antibody
Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 ug/ml.
Western Blot (WB) of anti-APCL antibody
Western blot of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 ug/ml.
NCBI/Uniprot data below describe general gene information for APCL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005874.1
[Other Products]
NCBI GenBank Nucleotide #
NM_005883.2
[Other Products]
UniProt Primary Accession #
O95996
[Other Products]
UniProt Secondary Accession #
Q05BW4; Q9UBZ1; Q9UEM8; Q9UQJ8; Q9UQJ9; Q9Y632[Other Products]
UniProt Related Accession #
O95996[Other Products]
Molecular Weight
243,821 Da
NCBI Official Full Name
adenomatous polyposis coli protein 2
NCBI Official Synonym Full Names
adenomatosis polyposis coli 2
NCBI Official Symbol
APC2 [Similar Products]
NCBI Official Synonym Symbols
APCL
[Similar Products]
NCBI Protein Information
adenomatous polyposis coli protein 2; adenomatous polyposis coli like
UniProt Protein Name
Adenomatous polyposis coli protein 2
UniProt Synonym Protein Names
Adenomatous polyposis coli protein-like; APC-like
UniProt Gene Name
APC2 [Similar Products]
UniProt Synonym Gene Names
APCL; APC-like [Similar Products]
UniProt Entry Name
APC2_HUMAN
UniProt Comments for APCL
APCL: Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. Belongs to the adenomatous polyposis coli (APC) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: microtubule cytoskeleton; Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; actin filament; catenin complex
Molecular Function: protein binding; microtubule binding; beta-catenin binding
Biological Process: Wnt receptor signaling pathway
Research Articles on APCL
1. APCL uses the 15 amino acid repeats region of APC to target beta-catenin for degradation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
