Full Product Name
F12 siRNA (Rat)
Product Synonym Names
Coagulation factor XII; Hageman factor; HAF
Product Gene Name
F12 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for D3ZTE0
Specificity
F12 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat F12 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of F12 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
F12 sirna
siRNA to inhibit F12 expression using RNA interference
Applications Tested/Suitable for F12 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for F12. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001014028.1
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NCBI GenBank Nucleotide #
NM_001014006.1
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UniProt Primary Accession #
D3ZTE0
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UniProt Secondary Accession #
Q5M879[Other Products]
UniProt Related Accession #
D3ZTE0[Other Products]
Molecular Weight
65,844 Da
NCBI Official Full Name
coagulation factor XII
NCBI Official Synonym Full Names
coagulation factor XII (Hageman factor)
NCBI Official Symbol
F12 [Similar Products]
NCBI Official Synonym Symbols
HAF
[Similar Products]
NCBI Protein Information
coagulation factor XII
UniProt Protein Name
Coagulation factor XII
UniProt Synonym Protein Names
Hageman factor; HAF
Protein Family
Coagulation factor
UniProt Gene Name
F12 [Similar Products]
UniProt Synonym Gene Names
HAF [Similar Products]
UniProt Entry Name
FA12_RAT
UniProt Comments for F12
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa ().
Research Articles on F12
1. FXIIa-kininogen mediated vasoconstriction contributes significantly to CKD hypertension in Brown Norway rats.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
