Product Name
ARHGAP9, siRNA
Full Product Name
ARHGAP9 siRNA (Human)
Product Synonym Names
Rho GTPase-activating protein 9; Rho-type GTPase-activating protein 9
Product Gene Name
ARHGAP9 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BRR9
Specificity
ARHGAP9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ARHGAP9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARHGAP9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARHGAP9 sirna
siRNA to inhibit ARHGAP9 expression using RNA interference
Applications Tested/Suitable for ARHGAP9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARHGAP9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001073625.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001080156.1
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UniProt Primary Accession #
Q9BRR9
[Other Products]
UniProt Secondary Accession #
Q8NAF3; Q8TCJ3; Q8WYR0; Q96EZ2; Q96S74; B4DVI3; E9PDX9[Other Products]
UniProt Related Accession #
Q9BRR9[Other Products]
Molecular Weight
70,869 Da
NCBI Official Full Name
rho GTPase-activating protein 9 isoform 3
NCBI Official Synonym Full Names
Rho GTPase activating protein 9
NCBI Official Symbol
ARHGAP9 [Similar Products]
NCBI Official Synonym Symbols
10C; RGL1
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NCBI Protein Information
rho GTPase-activating protein 9
UniProt Protein Name
Rho GTPase-activating protein 9
UniProt Synonym Protein Names
Rho-type GTPase-activating protein 9
Protein Family
Rho GTPase-activating protein
UniProt Gene Name
ARHGAP9 [Similar Products]
UniProt Entry Name
RHG09_HUMAN
NCBI Summary for ARHGAP9
This gene encodes a member of the Rho-GAP family of GTPase activating proteins. The protein has substantial GAP activity towards several Rho-family GTPases in vitro, converting them to an inactive GDP-bound state. It is implicated in regulating adhesion of hematopoietic cells to the extracellular matrix. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ARHGAP9
ARHGAP9: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs; GAPs, Rac/Rho; Cell adhesion
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: cytosol
Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
Research Articles on ARHGAP9
1. The Ala370Ser polymorphism in the ARHGAP9 gene is associated with coronary artery spasm.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
