Full Product Name
Anti-ASAH2 Antibody
Product Gene Name
anti-ASAH2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant protein of human ASAH2
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ASAH2 antibody
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine.
Applications Tested/Suitable for anti-ASAH2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ASAH2 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.
NCBI Accession #
AAI07106.1
[Other Products]
UniProt Secondary Accession #
Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6[Other Products]
UniProt Related Accession #
Q9NR71[Other Products]
Molecular Weight
81,718 Da
NCBI Official Full Name
ASAH2 protein
NCBI Official Synonym Full Names
N-acylsphingosine amidohydrolase 2
NCBI Official Symbol
ASAH2 [Similar Products]
NCBI Official Synonym Symbols
HNAC1; BCDase; LCDase; NCDase; N-CDase
[Similar Products]
NCBI Protein Information
neutral ceramidase
UniProt Protein Name
Neutral ceramidase
UniProt Synonym Protein Names
Acylsphingosine deacylase 2; BCDase; LCDase; hCD
Protein Family
Neutral ceramidase
UniProt Gene Name
ASAH2 [Similar Products]
UniProt Synonym Gene Names
HNAC1; N-CDase; NCDase; hCD [Similar Products]
NCBI Summary for ASAH2
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
UniProt Comments for ASAH2
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5 (PubMed:10781606, PubMed:26190575). Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.
Research Articles on ASAH2
1. The structural basis for ceramide recognition and hydrolysis by human neutral ceramidase has been uncovered.
Precautions
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Disclaimer
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