Product Name
CHRNG, siRNA
Full Product Name
CHRNG siRNA (Human)
Product Synonym Names
ACHRG; Acetylcholine receptor subunit gamma
Product Gene Name
CHRNG sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07510
Specificity
CHRNG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHRNG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHRNG sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHRNG sirna
siRNA to inhibit CHRNG expression using RNA interference
Applications Tested/Suitable for CHRNG sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHRNG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005190.4
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NCBI GenBank Nucleotide #
NM_005199.4
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UniProt Primary Accession #
P07510
[Other Products]
UniProt Secondary Accession #
Q14DU4; Q53RG2; B3KWM8[Other Products]
UniProt Related Accession #
P07510[Other Products]
Molecular Weight
52,020 Da
NCBI Official Full Name
acetylcholine receptor subunit gamma
NCBI Official Synonym Full Names
cholinergic receptor, nicotinic, gamma (muscle)
NCBI Official Symbol
CHRNG [Similar Products]
NCBI Official Synonym Symbols
ACHRG
[Similar Products]
NCBI Protein Information
acetylcholine receptor subunit gamma
UniProt Protein Name
Acetylcholine receptor subunit gamma
Protein Family
Acetylcholine receptor
UniProt Gene Name
CHRNG [Similar Products]
UniProt Synonym Gene Names
ACHRG [Similar Products]
UniProt Entry Name
ACHG_HUMAN
NCBI Summary for CHRNG
The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in ***** skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009]
UniProt Comments for CHRNG
nAChRG: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNG are a cause of multiple pterygium syndrome lethal type (MUPSL). Multiple pterygia are found infrequently in children with arthrogryposis and in fetuses with fetal akinesia syndrome. In lethal multiple pterygium syndrome there is intrauterine growth retardation, multiple pterygia, and flexion contractures causing severe arthrogryposis and fetal akinesia. Subcutaneous edema can be severe, causing fetal hydrops with cystic hygroma and lung hypoplasia. Oligohydramnios and facial anomalies are frequent. Defects in CHRNG are a cause of multiple pterygium syndrome Escobar variant (MUPSE); also known as nonlethal type multiple pterygium syndrome. Escobar syndrome is a non-lethal form of arthrogryposis multiplex congenita. It is an autosomal recessive condition characterized by excessive webbing (pterygia), congenital contractures (arthrogryposis), and scoliosis. Variable other features include intrauterine death, congenital respiratory distress, short stature, faciocranial dysmorphism, ptosis, low-set ears, arachnodactyly and cryptorchism in males. Congenital contractures are common and may be caused by reduced fetal movements at sensitive times of development. Possible causes of decreased fetal mobility include space constraints such as oligohydramnion, drugs, metabolic conditions or neuromuscular disorders including myasthenia gravis. is a. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Gamma/CHRNG sub-subfamily.
Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction
Molecular Function: channel activity; acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity
Biological Process: synaptic transmission; regulation of membrane potential; muscle contraction; transport; signal transduction; transmembrane transport
Disease: Multiple Pterygium Syndrome, Lethal Type; Multiple Pterygium Syndrome, Escobar Variant
Research Articles on CHRNG
1. No mutations were found in CHRNG, CHRND and CHRNA1 genes of Indian families with Escobar syndrome.
Precautions
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Disclaimer
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