KLKB1, Polyclonal Antibody

Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; PKK; Plasma kallikrein heavy chain; Plasma kallikrein light chain; KLKB1; KLK3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

>95%, Protein G purified

Liquid

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-KLKB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

WB: 1: 500-1: 5000
IHC: 1: 20-1: 200

Western Blot
Positive WB detected in: HepG2 whole cell lysate,Rat liver tissue,Rat brain tissue,Mouse heart tissue
All lanes: KLKB1 antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 KDa
Observed band size: 72 KDa

Immunohistochemistry of paraffin-embedded human liver tissue using MBS7048528 at dilution of 1:100

71,370 Da

kallikrein B1

plasma kallikrein

Plasma kallikrein

KLK3; PKK  [Similar Products]

KLKB1_HUMAN

This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation, intrinsic pathway; extracellular matrix disassembly; Factor XII activation; plasminogen activation; positive regulation of fibrinolysis; proteolysis; zymogen activation

Disease: Prekallikrein Deficiency

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