ADAM15, Monoclonal Antibody

Anti -ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

For Research Use Only. Not for use in diagnostic procedures.

chromosome: 1; Location: 1q21.3

Monoclonal

IgG1

9H33

Mouse

Recognizes human ADAM15 ectodomain.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-ADAM15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Antibodies; Abs to Matrix Metalloproteinases

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction 200ul.
Immunocytochemistry: 10ug/ml. Cells fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. After blocking, cells were incubated with diluted A0859-31B followed by labeled anti-mouse IgG.
Western Blot: 1-2ug/ml. Detects ~5ng/lane of rhADAM15 under non-reducing conditions.
Immunoprecipitation: 25ug/ml
Direct ELISA: 1ug/ml detects ~0.3ng rhADAM15/well.

92,959 Da[Similar Products]

ADAM metallopeptidase domain 15

disintegrin and metalloproteinase domain-containing protein 15; MDC-15; ADAM 15; metargidin; OTTHUMP00000033236; metalloprotease RGD disintegrin protein; a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15

Disintegrin and metalloproteinase domain-containing protein 15

MDC15  [Similar Products]

ADA15_HUMAN

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cell projection; adherens junction; cell surface; acrosome; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; proteolysis; negative regulation of cell-matrix adhesion; collagen catabolic process; extracellular matrix disassembly; tissue regeneration; innate immune response; negative regulation of cell growth; angiogenesis; negative regulation of cell migration

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