AT-rich interactive domain-containing protein 1A (ARID1A), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ARID1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7211683 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AT-rich interactive domain-containing protein 1A (ARID1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ARID1A. The ELISA analytical biochemical technique of the MBS7211683 kit is based on ARID1A antibody-ARID1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ARID1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ARID1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

206,532 Da

AT rich interactive domain 1A (SWI-like)

AT-rich interactive domain-containing protein 1A; BAF250; Swi1 like; osa homolog 1; SWI-like protein; BRG1-associated factor 250; BRG1-associated factor 250a; SWI-SNF complex protein p270; SWI/SNF complex protein p270; ARID domain-containing protein 1A; AT rich interactive domain 1A (Swi1 like); SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1

AT-rich interactive domain-containing protein 1A

Baf250; Baf250a; Osa1; Smarcf1; ARID domain-containing protein 1A; BAF250; BAF250A  [Similar Products]

ARI1A_MOUSE

ARID1A: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their ***** state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Defects in ARID1A are the cause of mental retardation autosomal dominant type 14 (MRD14). A disease characterized by multiple congenital anomalies and mental retardation. Mental retardation is defined by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD14 patients manifest developmental delay, abnormal corpus callosum, absent/hypoplastic fifth finger/toenails, sparse scalp hair, long eyelashes, and a coarse facial appearance with wide mouth, thick lips, and abnormal ears. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; SWI/SNF complex; nuclear chromatin; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; DNA binding; nucleosome binding

Biological Process: nervous system development; establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; cardiac muscle cell differentiation; chromatin remodeling; nucleosome disassembly; regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; forebrain development; androgen receptor signaling pathway; neural tube closure; ATP-dependent chromatin remodeling; maintenance of chromatin silencing

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