Full Product Name
mAb anti-human Raf-1
Product Synonym Names
Raf-1; Mouse monoclonal antibody to human Raf-1
Product Gene Name
anti-Raf-1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human. Others not tested
Specificity
Reactive to Raf-1 N-terminal (1-240 a. a.)
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.2
Target Protein
Human Raf-1
Immunogen
Purified recombinant Raf-1 (mw:75kDa)
Reconstitution
Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.
Research Area
Cell differentiation, oncology
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-Raf-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Raf-1 antibody
Mouse monoclonal antibody to human raf-1, Protein G affinity purified
Product Categories/Family for anti-Raf-1 antibody
Primary antibody; Monoclonal Antibodies to Signal Transduction
Applications Tested/Suitable for anti-Raf-1 antibody
Western Blot (WB)
Application Notes for anti-Raf-1 antibody
Western Blot: The antibody, when used at 1mug/mL, will allow visualization of 0.2mug/lane of recombinant human Raf-1 (75 kDa).
NCBI/Uniprot data below describe general gene information for Raf-1. It may not necessarily be applicable to this product.
NCBI Accession #
AAA60247.1
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UniProt Secondary Accession #
Q15278; Q9UC20; B0LPH8; B2R5N3[Other Products]
UniProt Related Accession #
P04049[Other Products]
Molecular Weight
75,395 Da
NCBI Official Full Name
RAF1, partial
NCBI Official Synonym Full Names
Raf-1 proto-oncogene, serine/threonine kinase
NCBI Official Symbol
RAF1 [Similar Products]
NCBI Official Synonym Symbols
NS5; CRAF; Raf-1; c-Raf; CMD1NN
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NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1
UniProt Protein Name
RAF proto-oncogene serine/threonine-protein kinase
UniProt Synonym Protein Names
Proto-oncogene c-RAF; cRaf; Raf-1
UniProt Gene Name
RAF1 [Similar Products]
UniProt Entry Name
RAF1_HUMAN
NCBI Summary for Raf-1
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
UniProt Comments for Raf-1
RAF1: a proto-oncogenic TKL kinase of the RAF family. Functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate and activate MEK1/2, which in turn phosphorylate and activate ERK1/2. Acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MEK1/2) and the extracellular signal-regulated kinases (ERK1/2). RAF1, subsequent to phosphorylation by PAK1, phosphorylates BAD (Bcl2-antagonist of cell death) at S75. Phosphorylates adenylyl cyclases ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2 (cardiac muscle troponin T). Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (ASK1 and MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Protein kinase, TKL; EC 2.7.11.1; TKL group; RAF family
Chromosomal Location of Human Ortholog: 3p25
Cellular Component: Golgi apparatus; mitochondrial outer membrane; cytoplasm; plasma membrane; pseudopodium; cytosol; nucleus
Molecular Function: small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; protein heterodimerization activity; mitogen-activated protein kinase kinase binding; metal ion binding; ATP binding; protein kinase activity
Biological Process: axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; wound healing; somatic stem cell maintenance; apoptosis; heart development; intermediate filament cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; protein amino acid phosphorylation; regulation of apoptosis; negative regulation of cell proliferation; synaptic transmission; small GTPase mediated signal transduction; regulation of cell differentiation; transmembrane transport; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; adenylate cyclase activation; MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; cell proliferation; Ras protein signal transduction; insulin receptor signaling pathway; response to hypoxia; regulation of Rho protein signal transduction; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; negative regulation of protein complex assembly; negative regulation of apoptosis
Disease: Cardiomyopathy, Dilated, 1nn; Noonan Syndrome 5; Leopard Syndrome 2
Research Articles on Raf-1
1. These data suggest that miR-7-5p functions as a tumor suppressor gene to regulate glioblastoma microvascular endothelial cell proliferation potentially by targeting the RAF1 oncogene
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