1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AGPAT3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7217630 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AGPAT3. The ELISA analytical biochemical technique of the MBS7217630 kit is based on AGPAT3 antibody-AGPAT3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AGPAT3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AGPAT3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

45,514 Da

1-acylglycerol-3-phosphate O-acyltransferase 3

1-acyl-sn-glycerol-3-phosphate acyltransferase gamma; 1-AGPAT 3; 1-AGP acyltransferase 3; lysophosphatidic acid acyltransferase gamma; lysophosphatidic acid acyltransferase-gamma1

1-acyl-sn-glycerol-3-phosphate acyltransferase gamma

LPAAT3; UNQ759/PRO1490; 1-AGP acyltransferase 3; 1-AGPAT 3; LPAAT-gamma  [Similar Products]

PLCC_HUMAN

The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

AGPAT3: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - ether lipid; Membrane protein, multi-pass; Membrane protein, integral; Lipid Metabolism - glycerolipid; EC 2.3.1.51; Lipid Metabolism - glycerophospholipid; Acetyltransferase

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; plasma membrane; integral to membrane; nuclear envelope

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process

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