Full Product Name
Anti-MYOD Antibody
Product Gene Name
anti-MYOD antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15172
Species Reactivity
Human, Mouse, Rat
Specificity
MyoD (phospho-Ser200) antibody detects endogenous levels of MyoD only when phosphorylated at serine 200.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
Concentration
1ug/ul (lot specific)
Storage Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MyoD around the phosphorylation site of serine 200 (A-S-SP-P-R).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-MYOD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MYOD antibody
Western Blot (WB)
Application Notes for anti-MYOD antibody
WB: 1:500~1:1000
NCBI/Uniprot data below describe general gene information for MYOD. It may not necessarily be applicable to this product.
NCBI Accession #
CAA40000.1
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UniProt Primary Accession #
P15172
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UniProt Secondary Accession #
O75321[Other Products]
UniProt Related Accession #
P15172[Other Products]
Molecular Weight
34,501 Da
NCBI Official Full Name
MyoD
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1 [Similar Products]
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
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NCBI Protein Information
myoblast determination protein 1; class C basic helix-loop-helix protein 1; myf-3; myogenic factor 3
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
UniProt Gene Name
MYOD1 [Similar Products]
UniProt Entry Name
MYOD1_HUMAN
NCBI Summary for MYOD
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for MYOD
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Protein type: RNA splicing; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; cytoplasm; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; myotube cell development; skeletal muscle fiber development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Product References and Citations for anti-MYOD antibody
Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776
Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24(4): 1809-1821.
Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19(4): 3167-3176.
Research Articles on MYOD
1. A recurring point mutation in MYOD1 is found in 10% of embryonal rhabdomyosarcomas with distinct clinical features and poor prognosis.
Precautions
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Disclaimer
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