Beta Tubulin, Antibody

beta 4; beta tubulin; MC1R; TUBB; tubulin

For Research Use Only. Not for use in diagnostic procedures.

IgG

Rabbit

>=95% as determined by SDS-PAGE
Immunogen Affinity Purified

Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500-1:2000
IHC: 1:20-1:200

Immunohistochemistry of paraffin-embedded human liver cancer using (beta Tubulin Antibody) at dilution of 1:50

HepG2 cells were subjected to SDS PAGE followed by western blot with (beta Tubulin antibody) at dilution of 1:1000

50-55 kDa [Similar Products]

tubulin beta 3 class III

tubulin beta-3 chain

Tubulin beta-3 chain

TUBB4  [Similar Products]

TBB3_HUMAN

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding

Biological Process: protein polymerization; axon guidance; mitosis; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1

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