Product Name
beta Tubulin 3 (TUBB3), Polyclonal Antibody
Full Product Name
beta Tubulin 3 antibody
Product Synonym Names
Polyclonal Beta Tubulin 3; Anti-Beta Tubulin 3; Tubulin 3; Beta-Tubulin 3; TUJ; B Tubulin
Product Gene Name
anti-TUBB3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13509
Purity/Purification
Beta Tubulin 3 antibody was purified by affinity chromatography.
Form/Format
Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration
100 ug/ml (lot specific)
Biological Significance
Tubulin is the major component of microtubules, essential cell cytoskeleton proteins involved in mitosis, cytokinesis and transport of vesicles and organelles. Tubulin is a dimer composed of one alpha and one beta tubulin molecule, which assemble as microtubules in a GTP-dependent manner. The beta-Tubulin molecule exerts GTPase activity to hydrolyze to GDP, effectively governing microtubule formation.
Immunogen
Beta Tubulin 3 antibody was raised in Chicken using two synthetic peptide (Human NP_AAL28094 and Rat AAM28438) / keyhole limpet hemocyanin (KLH) conjugates corresponding to different regions of the Beta Tubulin 3 gene product as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Protect from light.
Other Notes
Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBB3 antibody
Affinity purified Chicken polyclonal Beta Tubulin 3 antibody
Product Categories/Family for anti-TUBB3 antibody
Neuroscience; Purified Polyclonal Antibodies
Applications Tested/Suitable for anti-TUBB3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-TUBB3 antibody
ICC: 1:1000-1:2000
IHC: 1:1000-1:2000
WB: 1:2000-1:5000
Immunofluorescence of anti-TUBB3 antibody
Immunofluorescent staining of beta Tubulin 3 antibody
Dissociated cell culture of a neonatal mouse brain showing beta Tubulin 3 (green staining) of neurons. These cultures were counter-stained with a rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei.
NCBI/Uniprot data below describe general gene information for TUBB3. It may not necessarily be applicable to this product.
NCBI Accession #
AAL28094
[Other Products]
UniProt Primary Accession #
Q13509
[Other Products]
UniProt Secondary Accession #
Q9BTZ0; Q9BW10; A8K854[Other Products]
UniProt Related Accession #
Q13509[Other Products]
Molecular Weight
42,433 Da[Similar Products]
NCBI Official Full Name
class III beta tubulin
NCBI Official Synonym Full Names
tubulin, beta 3 class III
NCBI Official Symbol
TUBB3 [Similar Products]
NCBI Official Synonym Symbols
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
[Similar Products]
NCBI Protein Information
tubulin beta-3 chain
UniProt Protein Name
Tubulin beta-3 chain
UniProt Synonym Protein Names
Tubulin beta-4 chain; Tubulin beta-III
UniProt Gene Name
TUBB3 [Similar Products]
UniProt Synonym Gene Names
TUBB4 [Similar Products]
UniProt Entry Name
TBB3_HUMAN
NCBI Summary for TUBB3
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
UniProt Comments for TUBB3
TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; mitosis; axon guidance; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process
Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1
Research Articles on TUBB3
1. A mutation in a family provides new insights into the spectrum and variability of endocrine phenotypes associated with the TUBB3 E410K syndrome.">First report of an autosomal-dominant inheritance of the TUBB3 c.1228G>A mutation in a family provides new insights into the spectrum and variability of endocrine phenotypes associated with the TUBB3 E410K syndrome.
Precautions
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Disclaimer
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