Beta-tubulin, Monoclonal Antibody

TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

Mouse

Recognizes endogenous levels of Beta-tubulin protein.

Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal to Beta-tubulin

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Western Blot: (1/5000 - 1/10000); Immunohistochemistry: (1/100 - 1/200); Immunoprecipitation: (1/100 - 1/200)

Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.

Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using anti-Beta-tubulin Antibody.

42,433 Da

tubulin beta 3 class III

tubulin beta-3 chain

Tubulin beta-3 chain

TUBB4  [Similar Products]

TBB3_HUMAN

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: axon; cell soma; cytoplasm; dendrite; microtubule; nucleus

Molecular Function: GTP binding; GTPase activity; peptide binding; protein binding; structural constituent of cytoskeleton

Biological Process: 'de novo' posttranslational protein folding; axon guidance; cellular protein metabolic process; microtubule-based process; mitosis; protein folding

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 1; Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement

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