Full Product Name
MyoD Polyclonal Antibody
Product Synonym Names
MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Product Gene Name
anti-MyoD antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15172
Species Reactivity
Human, Mouse, Rat
Specificity
MyoD Polyclonal Antibody detects endogenous levels of MyoD protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Immunogen Description
Synthesized peptide derived from human MyoD around the non-acetylation site of K99/K102.
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-MyoD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MyoD antibody
Total protein Ab
Applications Tested/Suitable for anti-MyoD antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MyoD antibody
Western Blot: 1:500 - 1:2000
ELISA: 1:20000
Not yet tested in other applications.
Western Blot (WB) of anti-MyoD antibody
Western Blot analysis of Jurkat cells using MyoD Polyclonal Antibody
NCBI/Uniprot data below describe general gene information for MyoD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002469.2
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NCBI GenBank Nucleotide #
NM_002478.4
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UniProt Primary Accession #
P15172
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UniProt Secondary Accession #
O75321[Other Products]
UniProt Related Accession #
P15172[Other Products]
Molecular Weight
34,501 Da
NCBI Official Full Name
myoblast determination protein 1
NCBI Official Synonym Full Names
myogenic differentiation 1
NCBI Official Symbol
MYOD1 [Similar Products]
NCBI Official Synonym Symbols
PUM; MYF3; MYOD; bHLHc1
[Similar Products]
NCBI Protein Information
myoblast determination protein 1
UniProt Protein Name
Myoblast determination protein 1
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
UniProt Gene Name
MYOD1 [Similar Products]
UniProt Synonym Gene Names
BHLHC1; MYF3; MYOD; bHLHc1; Myf-3 [Similar Products]
UniProt Entry Name
MYOD1_HUMAN
NCBI Summary for MyoD
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for MyoD
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Protein type: DNA-binding; Transcription factor; RNA splicing
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; cytoplasm; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; chromatin DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; myotube cell development; skeletal muscle fiber development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on MyoD
1. MUNC is not a classic cis-acting enhancer RNA (e-RNA) acting exclusively by stimulating the neighboring MyoD gene
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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