Product Name
SLC16A2, siRNA
Full Product Name
SLC16A2 siRNA (Rat)
Product Synonym Names
MCT8; Monocarboxylate transporter 8; MCT 8; Solute carrier family 16 member 2
Product Gene Name
SLC16A2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8K1P8
Specificity
SLC16A2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC16A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC16A2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC16A2 sirna
siRNA to inhibit SLC16A2 expression using RNA interference
Applications Tested/Suitable for SLC16A2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC16A2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_671749.1
[Other Products]
NCBI GenBank Nucleotide #
NM_147216.1
[Other Products]
UniProt Primary Accession #
Q8K1P8
[Other Products]
UniProt Related Accession #
Q8K1P8[Other Products]
Molecular Weight
60,118 Da
NCBI Official Full Name
monocarboxylate transporter 8
NCBI Official Synonym Full Names
solute carrier family 16, member 2 (thyroid hormone transporter)
NCBI Official Symbol
Slc16a2 [Similar Products]
NCBI Protein Information
monocarboxylate transporter 8
UniProt Protein Name
Monocarboxylate transporter 8
UniProt Synonym Protein Names
Solute carrier family 16 member 2
Protein Family
Monocarboxylate transporter
UniProt Gene Name
Slc16a2 [Similar Products]
UniProt Synonym Gene Names
Mct8; MCT 8 [Similar Products]
UniProt Entry Name
MOT8_RAT
NCBI Summary for SLC16A2
human homolog is a monocarboxylate transporter [RGD, Feb 2006]
UniProt Comments for SLC16A2
SLC16A2: Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr. Defects in SLC16A2 are the cause of monocarboxylate transporter 8 deficiency (MCT8 deficiency); also known as Allan-Herndon-Dudley syndrome (AHDS). MCT8 deficiency consists of a severe form of X-linked psychomotor retardation combined with abnormal thyroid hormone (TH) levels. Thyroid hormone deficiency can be caused by defects of hormone synthesis and action, but it has also been linked to a defect in cellular hormone transport. Affected patients are males with abnormal relative concentrations of three circulating iodothyronines, as well as severe neurological abnormalities, including global developmental delay, central hypotonia, spastic quadriplegia, dystonic movements, rotary nystagmus, and impaired gaze and hearing. Heterozygous females had a milder thyroid phenotype and no neurological defects. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter
Cellular Component: plasma membrane; integral to membrane
Molecular Function: thyroid hormone transmembrane transporter activity; symporter activity
Biological Process: hormone transport; transmembrane transport
Research Articles on SLC16A2
1. the coordinated effects of Oatp1c1, Mct8, D2 and D3 in the placental barrier may regulate both transplacental TH passage and the development of trophoblast cells during thyroid dysfunction throughout the pregnancy.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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