Actin, gamma, Polyclonal Antibody

Anti -Actin, gamma

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_006097.3 (2014377..2015913). Location: chromosome: 10

Polyclonal

IgG

Sheep

Recognizes human gamma actin. Species Crossreactivity: rat and mouse. Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton.

Serum
Serum

Supplied as a liquid.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ACTG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Actin is a 43kD highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. There are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.

Antibodies; Abs to Actin Related Proteins

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:500-1:2000. Non-specific bands can occur when A0760-45 is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kD band
Immunocytochemistry: 1:500-1:2000. Cultured cells fixed with PFA, then permeabilized with methanol.
Immunohistochemistry: 1:500-1:2000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used

41,836 Da[Similar Products]

Actin, gamma 1

actin, cytoplasmic type 5; gamma-actin; cytoplasmic 2; gamma 1 propeptide; actin, cytoplasmic 2; actin, gamma 1 propeptide; actin, cytoplasmic, type 5

Actin, cytoplasmic type 5

ACT5_CHICK

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization

By similarity.

Sequence similarities: Belongs to the actin family.

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