Product Name
KCTD7, siRNA
Full Product Name
KCTD7 siRNA (Human)
Product Synonym Names
BTB/POZ domain-containing protein KCTD7
Product Gene Name
KCTD7 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96MP8
Specificity
KCTD7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCTD7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCTD7 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCTD7 sirna
siRNA to inhibit KCTD7 expression using RNA interference
Applications Tested/Suitable for KCTD7 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCTD7. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001161433.1
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NCBI GenBank Nucleotide #
NM_001167961.2
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UniProt Primary Accession #
Q96MP8
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UniProt Secondary Accession #
Q8IVR0; A4D2M4[Other Products]
UniProt Related Accession #
Q96MP8[Other Products]
Molecular Weight
32,946 Da
NCBI Official Full Name
BTB/POZ domain-containing protein KCTD7 isoform 2
NCBI Official Synonym Full Names
potassium channel tetramerization domain containing 7
NCBI Official Symbol
KCTD7 [Similar Products]
NCBI Official Synonym Symbols
EPM3; CLN14
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NCBI Protein Information
BTB/POZ domain-containing protein KCTD7
UniProt Protein Name
BTB/POZ domain-containing protein KCTD7
Protein Family
BTB/POZ domain-containing protein
UniProt Gene Name
KCTD7 [Similar Products]
UniProt Entry Name
KCTD7_HUMAN
NCBI Summary for KCTD7
This gene encodes a member of the potassium channel tetramerization domain-containing protein family. Family members are identified on a structural basis and contain an amino-terminal domain similar to the T1 domain present in the voltage-gated potassium channel. Mutations in this gene have been associated with progressive myoclonic epilepsy-3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]
UniProt Comments for KCTD7
KCTD7: contains a K+ channel tetramerisation domain (T1) found in voltage-gated K+ channel proteins. Encodes molecular determinants for the assembly of functional tetrameric channels. Two alternatively spliced isoforms have been described.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 7q11.21
Cellular Component: plasma membrane; cytosol
Biological Process: protein homooligomerization
Disease: Epilepsy, Progressive Myoclonic 3, With Or Without Intracellular Inclusions
Research Articles on KCTD7
1. This study identified that novel KCTD7 mutation in patients with progressive myoclonus epilepsy with ataxia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
