A kinase (PRKA) anchor protein 4, ELISA Kit

Human A-kinase anchor protein 4 (AKAP4) ELISA kit; RP13-377G1__B.1; AKAP82; CT99; FSC1; HI; hAKAP82; p82; A-kinase anchor protein 4; A-kinase anchor protein 82 kDa; cancer/testis antigen 99; protein kinase A anchoring protein 4; testis-specific gene HI; A kinase (PRKA) anchor protein 4

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human AKAP4. No significant cross-reactivity or interference between human AKAP4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of AKAP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS926560 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A kinase (PRKA) anchor protein 4 (AKAP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AKAP4. The ELISA analytical biochemical technique of the MBS926560 kit is based on AKAP4 antibody-AKAP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AKAP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AKAP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AKAP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AKAP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AKAP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AKAP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

94,477 Da

A kinase (PRKA) anchor protein 4

A-kinase anchor protein 4; testis-specific gene HI; cancer/testis antigen 99; A-kinase anchor protein 82 kDa; major sperm fibrous sheath protein; protein kinase A anchoring protein 4; protein kinase A-anchoring protein 4

A-kinase anchor protein 4

AKAP82; AKAP-4; AKAP 82; hAKAP82; HI; PRKA4  [Similar Products]

AKAP4_HUMAN

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex

Molecular Function: protein kinase A binding

Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; signal transduction; cell motility

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