14-3-3 epsilon, Polyclonal Antibody

14-3-3E; 143E; KCIP-1; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein-1; YWHAE

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

14-3-3 epsilon Antibody detects endogenous levels of total 14-3-3 epsilon protein.

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1 mg/ml (lot specific)

Stable at -20 degree C for at least 1 year.

Small volumes of anti-14-3-3 epsilon antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000
ELISA: 1:20000

Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is blocked with the synthesized peptide.

29 kDa

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon

14-3-3 protein epsilon

14-3-3 protein epsilon

14-3-3E  [Similar Products]

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326).

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