Product Name
ATIII - Antithrombin (Serpinc1), Polyclonal Antibody
Full Product Name
Sheep anti-human Antithrombin (ATIII), FITC-Conjugated Affinity-Purified IgG
Product Synonym Names
ATIII - Antithrombin, human
Product Gene Name
anti-Serpinc1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000067.5 (162908737..162933146). Location: 1 H2.1; 1 84.6 cM
3D Structure
ModBase 3D Structure for P32261
Specificity
Prior to conjugation, this antibody is specific for antithrombin as demonstrated by immunoelectrophoresis and ELISA.
Purity/Purification
Affinity purified, FITC conjugated IgG.
Vial containing mL of affinity-purified IgG conjugated to fluorescein isothiocyanate (FITC). Total protein is 0.1 mg.
Form/Format
APIgG-FITC conjugate as a clear yellow liquid.
Concentration
APIgG-FITC concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14. (lot specific)
Immunogen
Human antithrombin purified from plasma.
Buffer
Phosphate-buffered saline containing 1 mg/mL bovine albumin and 0.1% sodium azide, pH 7.4.
Preparation and Storage
Store at 2 degree C to 8 degree C and protect from light.
Other Notes
Small volumes of anti-Serpinc1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Serpinc1 antibody
Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 ug/mL (~3.5 uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa1-3.
Application Notes for anti-Serpinc1 antibody
Suitable as a source of fluorescein labelled antibodies to antithrombin.
NCBI/Uniprot data below describe general gene information for Serpinc1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
P32261
[Other Products]
UniProt Related Accession #
P32261; Q543J5[Other Products]
Molecular Weight
52,004 Da[Similar Products]
NCBI Official Full Name
antithrombin III
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol
Serpinc1 [Similar Products]
NCBI Official Synonym Symbols
At3; At-3; ATIII; AI114908
[Similar Products]
NCBI Protein Information
antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
UniProt Protein Name
Antithrombin-III
UniProt Synonym Protein Names
Serpin C1
Protein Family
Antithrombin
UniProt Gene Name
Serpinc1 [Similar Products]
UniProt Synonym Gene Names
At3 [Similar Products]
UniProt Entry Name
ANT3_MOUSE
UniProt Comments for Serpinc1
Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Subcellular location: Secreted › extracellular space.
Tissue specificity: Plasma.
Post-translational modification: Phosphorylation sites are present in the extracelllular medium
Sequence similarities: Belongs to the serpin family.
Product References and Citations for anti-Serpinc1 antibody
1. Damus PS, Rosenberg RD; Antithrombin - Heparin Cofactor; Methods in Enzymology 45, pp 653-669, 1976.
2. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.
3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.
Research Articles on Serpinc1
1. Mice homozygous for the heparin-binding domain mutation R48C developed spontaneous, life-threatening thrombosis, occurring as early as the day of birth. Only 60% of the offspring reached weaning age, with further loss at different ages
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Disclaimer
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