Product Name
HESX1, siRNA
Full Product Name
HESX1 siRNA (Human)
Product Synonym Names
HANF; Homeobox expressed in ES cells 1; Homeobox protein ANF; hAnf
Product Gene Name
HESX1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UBX0
Specificity
HESX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HESX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HESX1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HESX1 sirna
siRNA to inhibit HESX1 expression using RNA interference
Applications Tested/Suitable for HESX1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HESX1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003856.1
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NCBI GenBank Nucleotide #
NM_003865.2
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UniProt Primary Accession #
Q9UBX0
[Other Products]
UniProt Secondary Accession #
Q52LC5; Q99667[Other Products]
UniProt Related Accession #
Q9UBX0[Other Products]
Molecular Weight
21,409 Da
NCBI Official Full Name
homeobox expressed in ES cells 1
NCBI Official Synonym Full Names
HESX homeobox 1
NCBI Official Symbol
HESX1 [Similar Products]
NCBI Official Synonym Symbols
ANF; RPX; CPHD5
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NCBI Protein Information
homeobox expressed in ES cells 1
UniProt Protein Name
Homeobox expressed in ES cells 1
UniProt Synonym Protein Names
Homeobox protein ANF; hAnf
Protein Family
Homeobox expressed in ES cells
UniProt Gene Name
HESX1 [Similar Products]
UniProt Synonym Gene Names
HANF; hAnf [Similar Products]
UniProt Entry Name
HESX1_HUMAN
NCBI Summary for HESX1
This gene encodes a conserved homeobox protein that is a transcriptional repressor in the developing forebrain and pituitary gland. Mutations in this gene are associated with septooptic dysplasia, HESX1-related growth hormone deficiency, and combined pituitary hormone deficiency. [provided by RefSeq, Jul 2008]
UniProt Comments for HESX1
HESX1: Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation. Defects in HESX1 are a cause of septooptic dysplasia (SOD); also known as de Morsier syndrome. SOD is a rare autosomal recessive disease. SOD is characterized by optic nerve hypoplasia, absence of the corpus callosum and hypoplasia of the pituitary gland with panhypopopituitarism. Defects in HESX1 are a cause of growth hormone deficiency with pituitary anomalies (GHDPA). Defects in HESX1 are the cause of pituitary hormone deficiency combined type 5 (CPHD5). This disorder is manifested by deficiencies in anterior pituitary tropic hormones. week-old embryo. Belongs to the ANF homeobox family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 3p14.3
Cellular Component: nucleus
Molecular Function: protein C-terminus binding; protein binding; DNA binding; sequence-specific DNA binding; protein N-terminus binding; chromatin binding
Biological Process: transcription, DNA-dependent; brain development; otic vesicle formation; negative regulation of transcription, DNA-dependent; forebrain morphogenesis; nose development
Disease: Septooptic Dysplasia
Research Articles on HESX1
1. Data indicate that HESX1, LHX4 and SOX3 polymorphisms may be associated with pituitary stalk interruption syndrome (PSIS).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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