Product Name
KANSL1, siRNA
Full Product Name
KANSL1 siRNA (Human)
Product Synonym Names
KIAA1267; MSL1V1; NSL1; KAT8 regulatory NSL complex subunit 1; MLL1/MLL complex subunit KANSL1; MSL1 homolog 1; hMSL1v1; NSL complex protein NSL1; Non-specific lethal 1 homolog
Product Gene Name
KANSL1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7Z3B3
Specificity
KANSL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KANSL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KANSL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KANSL1 sirna
siRNA to inhibit KANSL1 expression using RNA interference
Applications Tested/Suitable for KANSL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KANSL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001180394.1
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NCBI GenBank Nucleotide #
NM_001193465.1
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UniProt Primary Accession #
Q7Z3B3
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UniProt Secondary Accession #
Q6AW85; Q8IYH1; Q9BRH0; Q9NTE7; Q9UFT0; Q9ULF3; A8K5E4[Other Products]
UniProt Related Accession #
Q7Z3B3[Other Products]
Molecular Weight
48,445 Da
NCBI Official Full Name
KAT8 regulatory NSL complex subunit 1 isoform 2
NCBI Official Synonym Full Names
KAT8 regulatory NSL complex subunit 1
NCBI Official Symbol
KANSL1 [Similar Products]
NCBI Official Synonym Symbols
KDVS; NSL1; MSL1v1; CENP-36; hMSL1v1; KIAA1267
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NCBI Protein Information
KAT8 regulatory NSL complex subunit 1
UniProt Protein Name
KAT8 regulatory NSL complex subunit 1
UniProt Synonym Protein Names
MLL1/MLL complex subunit KANSL1; MSL1 homolog 1; hMSL1v1; NSL complex protein NSL1; Non-specific lethal 1 homolog
Protein Family
KAT8 regulatory NSL complex
UniProt Gene Name
KANSL1 [Similar Products]
UniProt Synonym Gene Names
CENP-36; KIAA1267; MSL1V1; NSL1; hMSL1v1 [Similar Products]
UniProt Entry Name
KANL1_HUMAN
NCBI Summary for KANSL1
This gene encodes a nuclear protein that is a subunit of two protein complexes involved with histone acetylation, the MLL1 complex and the NSL1 complex. The corresponding protein in Drosophila interacts with K(lysine) acetyltransferase 8, which is also a subunit of both the MLL1 and NSL1 complexes. [provided by RefSeq, Jun 2012]
UniProt Comments for KANSL1
KANSL1: As part of the NSL complex it is involved in acetylation of nucleosomal histone H4 on several lysine residues and therefore may be involved in the regulation of transcription. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 17q21.31
Cellular Component: nucleoplasm; nucleus; histone acetyltransferase complex
Molecular Function: protein binding; histone acetyltransferase activity (H4-K16 specific)
Biological Process: establishment and/or maintenance of chromatin architecture
Disease: Koolen-de Vries Syndrome
Research Articles on KANSL1
1. findings show that de novo loss-of-function mutations in KANSL1 cause a full del(17q21.31) phenotype in 2 unrelated individuals that lack deletion at 17q21.31; findings indicate that 17q21.31 deletion syndrome is a monogenic disorder caused by haploinsufficiency of KANSL1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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