Tyrosyl DNA Phosphodiesterase 1 (TDP1), ELISA Kit

SCAN1; Tyr-DNA phosphodiesterase 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Tyrosyl DNA Phosphodiesterase 1 (TDP1).
No significant cross-reactivity or interference between Tyrosyl DNA Phosphodiesterase 1 (TDP1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TDP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2098010 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TDP1. The ELISA analytical biochemical technique of the MBS2098010 kit is based on TDP1 antibody-TDP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TDP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TDP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosyl DNA Phosphodiesterase 1 (TDP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosyl DNA Phosphodiesterase 1 (TDP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosyl DNA Phosphodiesterase 1 (TDP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosyl DNA Phosphodiesterase 1 (TDP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Enzyme & Kinase

68,632 Da

tyrosyl-DNA phosphodiesterase 1

tyrosyl-DNA phosphodiesterase 1

Tyrosyl-DNA phosphodiesterase 1

Tyr-DNA phosphodiesterase 1  [Similar Products]

TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.

Protein type: DNA repair, damage; EC 3.1.4.-; Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q32

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: double-stranded DNA binding; exonuclease activity; single-stranded DNA binding; tyrosyl-DNA phosphodiesterase activity

Biological Process: DNA repair; double-strand break repair; single strand break repair

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