Product Name
Tyrosyl-DNA Phosphodiesterase 1 (TDP1), Recombinant Protein
Full Product Name
Recombinant Human Tyrosyl-DNA Phosphodiesterase 1, Sf9
Product Synonym Names
Tyrosyl-DNA phosphodiesterase 1; Tyr-DNA phosphodiesterase 1; TDP1; FLJ11090; MGC104252; TDP1 Human, Sf9; Tyrosyl-DNA Phosphodiesterase 1 Human Recombinant, Sf9
Product Gene Name
TDP1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
ADP MSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH
3D Structure
ModBase 3D Structure for Q9NUW8
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE
Form/Format
Sterile filtered clear solution
Protein solution (0.25mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
Other Notes
Small volumes of TDP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TDP1 recombinant protein
Description: TDP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 617 amino acids (1-608) and having a molecular mass of 69.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). TDP1 is fused to 9 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Introduction: TDP1 belongs to the phospholipase D family and contains two PLD phosphodiesterase domains. TDP1 is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. TDP1 may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. Mutations in the TDP1 gene are linked to the disease spinocerebellar ataxia with axonal neuropathy (SCAN1).
Product Categories/Family for TDP1 recombinant protein
ENZYMES; Enzymes; Tyrosine Kinase; TDP1 Human, Sf9
NCBI/Uniprot data below describe general gene information for TDP1. It may not necessarily be applicable to this product.
NCBI Accession #
Q9NUW8.2
[Other Products]
UniProt Primary Accession #
Q9NUW8
[Other Products]
UniProt Secondary Accession #
Q2HXX4; Q86TV8; Q96BK7; Q9NZM7; Q9NZM8[Other Products]
UniProt Related Accession #
Q9NUW8[Other Products]
Molecular Weight
41,630 Da
NCBI Official Full Name
Tyrosyl-DNA phosphodiesterase 1
NCBI Official Synonym Full Names
tyrosyl-DNA phosphodiesterase 1
NCBI Official Symbol
TDP1 [Similar Products]
NCBI Protein Information
tyrosyl-DNA phosphodiesterase 1
UniProt Protein Name
Tyrosyl-DNA phosphodiesterase 1
Protein Family
Tyrosyl-DNA phosphodiesterase
UniProt Gene Name
TDP1 [Similar Products]
UniProt Synonym Gene Names
Tyr-DNA phosphodiesterase 1 [Similar Products]
NCBI Summary for TDP1
The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]
UniProt Comments for TDP1
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Research Articles on TDP1
1. We show that two genes, TDP1, a tyrosyl-DNA-phosphdiesterase, and TAF12, an RNA polymerase II TATA-box binding factor, cause CIN when overexpressed in human cells. Using SDL screens in yeast, we identified a set of genes that when deleted specifically kill cells with high levels of Tdp1
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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