Product Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (Cyp8b1), Recombinant Protein
Full Product Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
Product Gene Name
Cyp8b1 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-500aa; full length protein
Sequence
TLWCTVLGAL LTVVGCLCLS LLLRHRRPWE PPLDKGFVPW LGHSMAFRKN MFEFLKGMRA KHGDVFTVQL GGQYFTFVMD PLSFGPIIKN TEKALDFQSY AKELVLKVFG YQSVDGDHRM IHLASTKHLM GQGLEELNQA MLDSLSLVML GPKGSSLGAS SWCEDGLFHF CYRILFKAGF LSLFGYTKDK QQDLDEADEL FRKFRRFDFL FPRFVYSLLG PREWVEVSQL QRLFHQRLSV EQNLEKDGIS CWLGYMLQFL REQGIASSMQ DKFNFMMLWA SQGNTGPTCF WVLLFLLKHQ DAMKAVREEA TRVMGKARLE AKKSFTFTPS ALKHTPVLDS VMEESLRLCA TPTLLRVVQE DYVLKMASGQ EYQIRRGDKV ALFPYLSVHM DPDIHPEPTA FKYDRFLNPD GTRKVDFYKS GKKIHHYSMP WGSGVSKCPG RFFALSEMKT FVLLMIMYFD FKLVDPDIPV PPIDPRRWGF GTSQPSHEVR FLYRLKPVQ
3D Structure
ModBase 3D Structure for O88962
Host
Cell Free Expression
Form/Format
Liquid containing glycerol
Species
Mus musculus (Mouse)
Storage Buffer
Tris-based buffer, 50% glycerol.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Cyp8b1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Cyp8b1 recombinant protein
This is a recombinant transmembrane protein expressed in a cell-free expression system.
Product Categories/Family for Cyp8b1 recombinant protein
Transmembrane Protein
NCBI/Uniprot data below describe general gene information for Cyp8b1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034142.3
[Other Products]
NCBI GenBank Nucleotide #
NM_010012.3
[Other Products]
UniProt Primary Accession #
O88962
[Other Products]
UniProt Secondary Accession #
Q9R217[Other Products]
UniProt Related Accession #
O88962[Other Products]
Molecular Weight
57,706 Da
NCBI Official Full Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450, family 8, subfamily b, polypeptide 1
NCBI Official Symbol
Cyp8b1 [Similar Products]
NCBI Protein Information
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Protein Name
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Synonym Protein Names
7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase
Protein Family
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
UniProt Gene Name
Cyp8b1 [Similar Products]
UniProt Synonym Gene Names
Cyp12 [Similar Products]
UniProt Entry Name
CP8B1_MOUSE
UniProt Comments for Cyp8b1
CYP8B1: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. Belongs to the cytochrome P450 family.
Protein type: Membrane protein, integral; EC 1.14.13.95; Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis
Molecular Function: sterol 12-alpha-hydroxylase activity
Research Articles on Cyp8b1
1. Absence of Cyp8b1 results in improved glucose tolerance, insulin sensitivity, and beta-cell function, mediated by absence of cholic acid in Cyp8b1(-/-) mice.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
