Product Name
AMELX, siRNA
Full Product Name
AMELX siRNA (Rat)
Product Synonym Names
AMEL; AMGX; Amelogenin. X isoform; Leucine-rich amelogenin peptide; LRAP
Product Gene Name
AMELX sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P63278
Specificity
AMELX siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AMELX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AMELX sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AMELX sirna
siRNA to inhibit AMELX expression using RNA interference
Applications Tested/Suitable for AMELX sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AMELX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001258002.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001271073.1
[Other Products]
UniProt Primary Accession #
P63278
[Other Products]
UniProt Secondary Accession #
P45559; Q62945; Q63640; Q78E56[Other Products]
UniProt Related Accession #
P63278[Other Products]
Molecular Weight
21,868 Da
NCBI Official Full Name
amelogenin, X isoform isoform 2
NCBI Official Synonym Full Names
amelogenin, X-linked
NCBI Official Symbol
Amelx [Similar Products]
NCBI Official Synonym Symbols
Amel
[Similar Products]
NCBI Protein Information
amelogenin, X isoform
UniProt Protein Name
Amelogenin, X isoform
UniProt Synonym Protein Names
Leucine-rich amelogenin peptide; LRAP
Protein Family
Amelogenin
UniProt Gene Name
Amelx [Similar Products]
UniProt Synonym Gene Names
Amel; Amgx; LRAP [Similar Products]
UniProt Entry Name
AMELX_RAT
NCBI Summary for AMELX
may play a role in mineralization of tooth enamel matrix [RGD, Feb 2006]
UniProt Comments for AMELX
AMELX: Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel. Defects in AMELX are the cause of amelogenesis imperfecta type 1E (AI1E). A X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small. Belongs to the amelogenin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; Golgi apparatus; proteinaceous extracellular matrix; cell surface; endocytic vesicle; cytoplasm; basement membrane
Molecular Function: identical protein binding; growth factor activity; hydroxyapatite binding; structural constituent of tooth enamel
Biological Process: response to drug; cell proliferation; biomineral formation; cell adhesion; response to calcium ion; response to nutrient
Research Articles on AMELX
1. The aim of this study was to verify the expression, distribution and role of Rho GTPases in ameloblasts and odontoblasts during tooth development in correlation with actin and tubulin arrangements and amelogenin and dentin sialophosphoprotein expression.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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