Full Product Name
TMC6 siRNA (Human)
Product Synonym Names
EVER1; EVIN1; Transmembrane channel-like protein 6; Epidermodysplasia verruciformis protein 1; Protein LAK-4
Product Gene Name
TMC6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7Z403
Specificity
TMC6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TMC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TMC6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TMC6 sirna
siRNA to inhibit TMC6 expression using RNA interference
Applications Tested/Suitable for TMC6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TMC6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001120670.1
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NCBI GenBank Nucleotide #
NM_001127198.1
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UniProt Primary Accession #
Q7Z403
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UniProt Secondary Accession #
O43284; Q45VJ2; Q8IU98; Q8IUI7; Q8IWU8; Q8TEQ7; Q9HAG5[Other Products]
UniProt Related Accession #
Q7Z403[Other Products]
Molecular Weight
32,485 Da
NCBI Official Full Name
transmembrane channel-like protein 6
NCBI Official Synonym Full Names
transmembrane channel-like 6
NCBI Official Symbol
TMC6 [Similar Products]
NCBI Official Synonym Symbols
EV1; EVER1; EVIN1; LAK-4P
[Similar Products]
NCBI Protein Information
transmembrane channel-like protein 6
UniProt Protein Name
Transmembrane channel-like protein 6
UniProt Synonym Protein Names
Epidermodysplasia verruciformis protein 1; Protein LAK-4
Protein Family
Transmembrane channel-like protein
UniProt Gene Name
TMC6 [Similar Products]
UniProt Synonym Gene Names
EVER1; EVIN1 [Similar Products]
UniProt Entry Name
TMC6_HUMAN
NCBI Summary for TMC6
Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 10 transmembrane domains and 2 leucine zipper motifs. [provided by RefSeq, Jul 2008]
UniProt Comments for TMC6
TMC6: Defects in TMC6 are a cause of epidermodysplasia verruciformis (EV). It is a rare autosomal recessive genodermatosis associated with a high risk of skin carcinoma that results from an abnormal susceptibility to infection by specific human papillomaviruses. Infection leads to persistent wart-like or macular lesions. Belongs to the TMC family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; integral to membrane
Molecular Function: protein binding
Biological Process: ion transport
Disease: Epidermodysplasia Verruciformis
Research Articles on TMC6
1. Expression of both EVER1 and EVER2 in B cells is activated immediately after Epstein-Barr virus (EBV) infection, whereas at later stages, it is strongly repressed by latent membrane protein 1-activated NF-kappaB signaling.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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