ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum, Monoclonal Ant

Anti -ATPase 2, Ca2+ Transporting, Sarcoplasmic Endoplasmic Reticulum (SERCA2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_010456.3 (35115998..35185461). Location: 14; 14

Monoclonal

IgG1

6D141

Mouse

Recognizes canine SERCA2a (sarcoplasmic/endoplasmic reticulum calcium ATPase 2a) and SERCA2b isoforms. Species crossreactivity: human, rat, porcine, bovine, rat. Does not crossreact with frog SERCA2 ATPase.

Ascites
Ascites

Supplied as a liquid in PBS, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ATP2A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Antibodies; Abs to Enzymes, ATPase

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (frozen sections): 1:500
Western Blot: 1:2500 Detects a band of ~110kD
Note: For detection of rat SERCA2 ATPase use A4000-93A.

114,792 Da[Similar Products]

sarcoplasmic/endoplasmic reticulum calcium ATPase 2; calcium pump 2; SR Ca(2+)-ATPase 2; Ca(2+)-transport ATPase class 3; endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic-reticulum Ca(2+) pump gene 2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

AT2A2_PIG

Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform SERCA2A is involved in the regulation of the contraction/relaxation cycle

Catalytic activity: ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Enzyme regulation: Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN

Subunit structure: Associated with phospholamban (PLN)

By similarity. Isoform SERCA2B interacts with TRAM2 (via C-terminus). Interacts with HAX1

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue specificity: Isoform SERCA2A is highly expressed in heart and slow twitch skeletal muscle. Isoform SERCA2B is widely expressed. Ref.3

Post-translational modification: Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity

Sequence similarities: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. [View classification]

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