Full Product Name
Anti-PRPF3 Antibody
Product Gene Name
anti-PRPF3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Specificity
Western blot analysis of extracts of various cell lines, using PRPF3 antibody. Immunofluorescence analysis of U20S cell using PRPF3 antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant protein of human PRPF3
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-PRPF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRPF3 antibody
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
Applications Tested/Suitable for anti-PRPF3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-PRPF3 antibody
WB: 1:500 - 1:2000
IHC: 1:50- 1:200
IF: 1:50- 1:200
Western Blot (WB) of anti-PRPF3 antibody
Immunofluorescence (IF) of anti-PRPF3 antibody
NCBI/Uniprot data below describe general gene information for PRPF3. It may not necessarily be applicable to this product.
NCBI Accession #
AKI70140.1
[Other Products]
UniProt Secondary Accession #
O43446; Q5VT54; B4DSY9[Other Products]
UniProt Related Accession #
O43395[Other Products]
Molecular Weight
31,889 Da
NCBI Official Full Name
PRPF3, partial
NCBI Official Synonym Full Names
pre-mRNA processing factor 3
NCBI Official Symbol
PRPF3 [Similar Products]
NCBI Official Synonym Symbols
PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
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NCBI Protein Information
U4/U6 small nuclear ribonucleoprotein Prp3
UniProt Protein Name
U4/U6 small nuclear ribonucleoprotein Prp3
UniProt Synonym Protein Names
Pre-mRNA-splicing factor 3; hPrp3; U4/U6 snRNP 90 kDa protein
Protein Family
U4/U6 small nuclear ribonucleoprotein
UniProt Gene Name
PRPF3 [Similar Products]
UniProt Synonym Gene Names
HPRP3; PRP3; hPrp3 [Similar Products]
NCBI Summary for PRPF3
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]
UniProt Comments for PRPF3
PRPF3: Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. Defects in PRPF3 are the cause of retinitis pigmentosa type 18 (RP18). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP18 inheritance is autosomal dominant.
Protein type: RNA processing; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: Cajal body; cytoplasm; nucleoplasm; nucleus; U4/U6 x U5 tri-snRNP complex
Molecular Function: identical protein binding; protein binding
Biological Process: assembly of spliceosomal tri-snRNP; mRNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing; RNA splicing, via transesterification reactions
Disease: Retinitis Pigmentosa 18
Research Articles on PRPF3
1. RP-PRPF defects affect the stoichiometry of spliceosomal small nuclear RNAs. Mutant PRPF3 proteins stably associated with tri-snRNPs.
Precautions
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