Product Name
Alpha Internexin/ NF-66 (INA), Monoclonal Antibody
Full Product Name
Anti-Alpha-Internexin (NF66)
Product Gene Name
anti-INA antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_005100.3 (274212015..274224831). Location: 1q54
3D Structure
ModBase 3D Structure for P23565
Species Reactivity
Most mammalian
Specificity
Specific for the ~66k alpha Internexin protein. Can be used on formalinfixed cells in tissue culture, cryostat sections, and Western blotting. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.
Purity/Purification
Total IgG fraction
Form/Format
Liquid containing 10mM sodium azide.
Antigen
Recombinant rat alpha-internexin expressed in and purified from E. coli.
Immunogen Information
Recombinant rat alpha-internexin expressed in and purified from E. coli
Species Reactivity Note
The antibody has been directly tested for reactivity in a wide variety of mammalian species.
Biological Significance
Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-INA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-INA antibody
Mouse monoclonal antibody
Applications Tested/Suitable for anti-INA antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-INA antibody
Quality Control: Western blots performed on each lot.
WB: 1:2,000
IHC: 1:1,000
IF: 1:250
Testing Data of anti-INA antibody
NCBI/Uniprot data below describe general gene information for INA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062001.1
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NCBI GenBank Nucleotide #
NM_019128.4
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UniProt Primary Accession #
P23565
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UniProt Related Accession #
P23565[Other Products]
NCBI Official Full Name
alpha-internexin
NCBI Official Synonym Full Names
internexin neuronal intermediate filament protein, alpha
NCBI Official Symbol
Ina [Similar Products]
NCBI Official Synonym Symbols
Nf66; Inexa; Intlaa
[Similar Products]
NCBI Protein Information
alpha-internexin; alpha-Inx; internexin, alpha
UniProt Protein Name
Alpha-internexin
Protein Family
Alpha-internexin
UniProt Gene Name
Ina [Similar Products]
UniProt Synonym Gene Names
Inexa; Alpha-Inx [Similar Products]
UniProt Entry Name
AINX_RAT
NCBI Summary for INA
may play a role in neurogenesis; may be involved in neuronal regeneration in response to injury [RGD, Feb 2006]
UniProt Comments for INA
Function: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges
By similarity.
Developmental stage: Levels of this protein reach a maximum at embryonic day 16 and decline into *****hood.
Post-translational modification: O-glycosylated
By similarity.
Sequence similarities: Belongs to the intermediate filament family.
Product References and Citations for anti-INA antibody
• Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985).
• McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002).
• Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004).
• Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004).
• Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).
Research Articles on INA
1. Recombinant rat alpha-internexin coassembles with all three neurofilament proteins into a single network of filaments in quadruple-transfected SW13vim(-) mouse cells.
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