U4/U6 small nuclear ribonucleoprotein Prp3 (PRPF3), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PRPF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7220579 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the U4/U6 small nuclear ribonucleoprotein Prp3 (PRPF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRPF3. The ELISA analytical biochemical technique of the MBS7220579 kit is based on PRPF3 antibody-PRPF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRPF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRPF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Epigenetics and Nuclear Signaling

77,529 Da

pre-mRNA processing factor 3

U4/U6 small nuclear ribonucleoprotein Prp3; U4/U6 snRNP 90 kDa protein; pre-mRNA-splicing factor 3; U4/U6-associated RNA splicing factor; PRP3 pre-mRNA processing factor 3 homolog

U4/U6 small nuclear ribonucleoprotein Prp3

HPRP3; PRP3; hPrp3  [Similar Products]

PRPF3_HUMAN

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]

PRPF3: Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. Defects in PRPF3 are the cause of retinitis pigmentosa type 18 (RP18). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP18 inheritance is autosomal dominant.

Protein type: RNA splicing; RNA processing; Spliceosome

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; spliceosome; Cajal body; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus

Molecular Function: identical protein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; mRNA processing

Disease: Retinitis Pigmentosa 18

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