Full Product Name
Phospho-NBN-S343 Polyclonal Antibody
Product Synonym Names
Phospho-NBN-S343: ATV; NBS; P95; NBS1; AT-V1; AT-V2
Product Gene Name
anti-NBN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60934
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
A phospho specific peptide corresponding to residues surrounding S343 of human NBN
Calculated Molecular Weight
95kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-NBN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NBN antibody
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Product Categories/Family for anti-NBN antibody
Phosphorylation
Applications Tested/Suitable for anti-NBN antibody
Western Blot (WB)
Application Notes for anti-NBN antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-NBN antibody
Western blot analysis of extracts from HeLa cells, using Phospho-NBN-S343 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
NCBI/Uniprot data below describe general gene information for NBN. It may not necessarily be applicable to this product.
NCBI Accession #
O60934.1
[Other Products]
UniProt Primary Accession #
O60934
[Other Products]
UniProt Secondary Accession #
O60672; Q32NF7; Q53FM6; Q63HR6; Q7LDM2; B2R626; B2RNC5[Other Products]
UniProt Related Accession #
O60934[Other Products]
NCBI Official Full Name
Nibrin
NCBI Official Synonym Full Names
nibrin
NCBI Official Symbol
NBN [Similar Products]
NCBI Official Synonym Symbols
ATV; NBS; P95; NBS1; AT-V1; AT-V2
[Similar Products]
NCBI Protein Information
nibrin; cell cycle regulatory protein p95; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex
UniProt Protein Name
Nibrin
UniProt Synonym Protein Names
Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1
UniProt Gene Name
NBN [Similar Products]
UniProt Synonym Gene Names
NBS; NBS1; P95 [Similar Products]
UniProt Entry Name
NBN_HUMAN
NCBI Summary for NBN
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
UniProt Comments for NBN
NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.
Protein type: DNA repair, damage; Cell cycle regulation
Chromosomal Location of Human Ortholog: 8q21
Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body
Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding
Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; isotype switching; positive regulation of kinase activity; DNA repair; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; DNA damage checkpoint; blastocyst growth; neuromuscular process controlling balance; cell cycle arrest; telomere maintenance
Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia
Research Articles on NBN
1. The results suggest that DNMT1 function in the regulatory response is controlled by NBS1.
Precautions
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Disclaimer
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