NBN, Polyclonal Antibody

Nibrin; Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1; NBN; NBS; NBS1; P95

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This NBN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-630 amino acids from the C-terminal region of human NBN.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.27 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-NBN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mutations in this gene are associated with Nijmegen
breakage syndrome, an autosomal recessive chromosomal instability
syndrome characterized by microcephaly, growth retardation,
immunodeficiency, and cancer predisposition. The encoded protein is
a member of the MRE11/RAD50 double-strand break repair complex
which consists of 5 proteins. This gene product is thought to be
involved in DNA double-strand break repair and DNA damage-induced
checkpoint activation.

Cancer

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

WB~~1:1000

NBN Antibody (C-term) western blot analysis in NCI-H460,NCI-H292 cell line lysates (35ug/lane).This demonstrates the NBN antibody detected the NBN protein (arrow).

Confocal immunofluorescent analysis of NBN Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

NBN Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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nibrin

nibrin

Nibrin

NBS; NBS1; P95  [Similar Products]

NBN_HUMAN

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body

Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; isotype switching; positive regulation of kinase activity; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; meiotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance

Disease: Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome; Aplastic Anemia

Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Kavitha, C.V., et al. Biochem. Biophys. Res. Commun. 399(4):575-580(2010)
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Jelonek, K., et al. J. Appl. Genet. 51(3):343-352(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

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