Full Product Name
MIWI Antibody
Product Synonym Names
Piwil1; Miwi; PIWIL1
Product Gene Name
anti-MIWI antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JMB7
Species Reactivity
Mouse, Rat
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of mouseMIWI
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-MIWI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MIWI antibody
The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
Product Categories/Family for anti-MIWI antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-MIWI antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-MIWI antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:20-1:100
IP: 1:20-1:100
Western Blot (WB) of anti-MIWI antibody
Western blot analysis of extracts of various celllines, using MIWI antibody.
NCBI/Uniprot data below describe general gene information for MIWI. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067286.1
[Other Products]
NCBI GenBank Nucleotide #
NM_021311.3
[Other Products]
UniProt Primary Accession #
Q9JMB7
[Other Products]
UniProt Secondary Accession #
Q6NXX0; A1L324; A1L325[Other Products]
UniProt Related Accession #
Q9JMB7[Other Products]
NCBI Official Full Name
piwi-like protein 1
NCBI Official Synonym Full Names
piwi-like RNA-mediated gene silencing 1
NCBI Official Symbol
Piwil1 [Similar Products]
NCBI Official Synonym Symbols
MIWI
[Similar Products]
NCBI Protein Information
piwi-like protein 1
UniProt Protein Name
Piwi-like protein 1
UniProt Gene Name
Piwil1 [Similar Products]
UniProt Comments for MIWI
Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:22121019, PubMed:21237665). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:22121019, PubMed:21237665). Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:11578866, PubMed:22121019, PubMed:21237665). Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias) (PubMed:24757166). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle (PubMed:22121019). Acts as an endoribonuclease that cleaves transposon messenger RNAs (PubMed:22121019). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:16938833). Probable component of some RISC complex, which mediates RNA cleavage and translational silencing (PubMed:16938833). Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (PubMed:16787948). Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1 (PubMed:28552346).
Product References and Citations for anti-MIWI antibody
1. Cox, D.N. et al. (1998) Genes Dev 12, 3715-27. 2. Kuramochi-Miyagawa, S. et al. (2001) Mech Dev 108, 121-33. 3. Aravin, A. et al. (2006) Nature 442, 203-7. 4. Grivna, S.T. et al. (2006) Proc Natl Acad Sci USA 103, 13415-20. 5. Grivna, S.T. et al. (2006) Genes Dev 20, 1709-14.
Research Articles on MIWI
1. MIWI (or PIWIL1) depletion had a smaller effect. In oocytes lacking PLD6 (or ZUCCHINI or MITOPLD), a mitochondrial nuclease/phospholipase involved in piRNA biogenesis in male germ cells, the piRNA level was decreased to 50% compared to wild-type.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
