Product Name
ENTPD6, siRNA
Full Product Name
ENTPD6 siRNA (Human)
Product Synonym Names
CD39L2; IL6ST2; Ectonucleoside triphosphate diphosphohydrolase 6; NTPDase 6; CD39 antigen-like 2
Product Gene Name
ENTPD6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O75354
Specificity
ENTPD6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ENTPD6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ENTPD6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ENTPD6 sirna
siRNA to inhibit ENTPD6 expression using RNA interference
Applications Tested/Suitable for ENTPD6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ENTPD6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107561.1
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NCBI GenBank Nucleotide #
NM_001114089.1
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UniProt Primary Accession #
O75354
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UniProt Secondary Accession #
Q5QPJ2; Q5QPJ5; Q7Z5B5; Q8N3H3; Q8TAS7; Q9UJD1; A6NCX6; D3DW49[Other Products]
UniProt Related Accession #
O75354[Other Products]
Molecular Weight
53,118 Da
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 6 isoform 2
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 6 (putative)
NCBI Official Symbol
ENTPD6 [Similar Products]
NCBI Official Synonym Symbols
CD39L2; IL6ST2; IL-6SAG; NTPDase-6; dJ738P15.3
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NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 6
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 6
UniProt Synonym Protein Names
CD39 antigen-like 2
Protein Family
Ectonucleoside triphosphate diphosphohydrolase
UniProt Gene Name
ENTPD6 [Similar Products]
UniProt Synonym Gene Names
CD39L2; IL6ST2; NTPDase 6 [Similar Products]
UniProt Entry Name
ENTP6_HUMAN
NCBI Summary for ENTPD6
ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which is characteristic of NTPases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ENTPD6
ENTPD6: Might support glycosylation reactions in the Golgi apparatus and, when released from cells, might catalyze the hydrolysis of extracellular nucleotides. Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent, there is no hydrolysis of nucleoside 5'-monophosphates. The order of activity with different substrates is GDP > IDP >> UDP = CDP >> ADP. Belongs to the GDA1/CD39 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.1.6; Nucleotide Metabolism - pyrimidine; Membrane protein, integral; Nucleotide Metabolism - purine; Hydrolase
Chromosomal Location of Human Ortholog: 20p11.21
Cellular Component: Golgi membrane; extracellular space; cell surface; plasma membrane; integral to membrane
Molecular Function: nucleoside-triphosphatase activity; guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity; uridine-diphosphatase activity
Biological Process: response to magnesium ion; metabolic process; response to calcium ion
Research Articles on ENTPD6
1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
