Full Product Name
MOUSE ANTI HUMAN CD32:Biotin
Product Gene Name
anti-CD32 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
Immunogen
K562 cell line
Fusion Partners
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-CD32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD32 antibody
Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a 40kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).
Applications Tested/Suitable for anti-CD32 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD32 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Maximum Dilution: Neat
Testing Data of anti-CD32 antibody
Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin (MBS210308)
Testing Data of anti-CD32 antibody
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:FITC (MBS210611)
Testing Data of anti-CD32 antibody
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:RPE (MBS213549)
Testing Data of anti-CD32 antibody
Staining of human peripheral blood granulocytes with Mouse anti Human CD32 (MBS212021)
Testing Data of anti-CD32 antibody
Staining of human peripheral blood granulocytes with Mouse anti Human CD32:Alexa Fluor 647 (MBS212021A647)
NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001129691.1
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NCBI GenBank Nucleotide #
NM_001136219.1
[Other Products]
UniProt Secondary Accession #
Q8WUN1; Q8WW64[Other Products]
UniProt Related Accession #
P12318[Other Products]
Molecular Weight
34,930 Da
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIa, receptor (CD32)
NCBI Official Symbol
FCGR2A [Similar Products]
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
[Similar Products]
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a; Immunoglobulin G Fc receptor II; fc-gamma RII-a; fc-gamma-RIIa; fcRII-a; igG Fc receptor II-a
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD_antigen: CD32
Protein Family
CD320 antigen
UniProt Gene Name
FCGR2A [Similar Products]
UniProt Synonym Gene Names
CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a [Similar Products]
UniProt Entry Name
FCG2A_HUMAN
NCBI Summary for CD32
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
UniProt Comments for CD32
FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.
Protein type: Membrane protein, integral; Receptor, misc.; Cell surface
Chromosomal Location of Human Ortholog: 1q23
Cellular Component: integral to membrane; plasma membrane
Molecular Function: IgG binding
Biological Process: innate immune response
Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To
Research Articles on CD32
1. This study describes a novel Q27W FcgammaRIIa variant that causes delayed downstream signaling in two common variable immune deficiency patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
