CD32A, Monoclonal Antibody

Anti-CD32A Antibody (clone 13D7) IHC-plus; FCGR2A; CD32; CD32 antigen; CDw32; FCG2; FcGR; FCGR2A1; FcRII-a; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-c; IgG Fc receptor II-a; Igg fc receptor ii-c; Fc-gamma rii-c; FCGR2; Igg fc receptors ii-c; CD32A; IGFR2; Human CD32A

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b

13D7

Mouse

Human CD32A / CD32

Protein A/G Purified

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

1.09 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-CD32A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)

IF (1:100), IHC-P (10 ug/ml), IP, WB (1:2000)
Usage: Concentration: 0.5-1.0 mg/ml (Lot dependent)

Anti-CD32A antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Anti-CD32A antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-FCGR2A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.

Immunoprecipitation(IP) of FCGR2A by using monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-FCGR2A antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

34,930 Da

Fc fragment of IgG, low affinity IIa, receptor (CD32)

low affinity immunoglobulin gamma Fc region receptor II-a; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; Immunoglobulin G Fc receptor II

Low affinity immunoglobulin gamma Fc region receptor II-a

CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a  [Similar Products]

FCG2A_HUMAN

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: IgG binding

Biological Process: innate immune response

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To

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