Crisp3, Monoclonal Antibody

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

LV-2A2

Mouse

anti-human cysteine-rich secretory protein 3 (Crisp3, SGP28)

Protein G

Purified

Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)

Small volumes of anti-CRISP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: Cysteine-rich secretory protein 3 (CRISP3) belongs to the cysteine-rich secretory protein family. CRISPs are characterized by a cysteine-rich domain at the COOH terminal that form 8 intramolecular disulfide bonds (1). Mammalian members of the CRISP family are expressed predominantly in the male reproductive tract and are implicated in the process of reproduction from spermiogenesis, posttesticular sperm maturation and capacitation to oocyte-sperm fusion (2). CRISP3 is epithelium-specific and found predominantly in salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. CRISP3 is up-regulated in malignant prostatic epithelium, therefore, it can be used as a potential prostate cancer biomarker (3).

Flow Cytometry (FC/FACS), CELISA

Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400

Fig.1: FACS analysis of BOSC23 cells using LV-2A2 Cat.# GM-0904. BOSC23 cells were transiently transfected with an expression vector encoding either Crisp3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LV-2A2 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with Crisp3 transfected cells.

Fig.2: SDS-PAGE analysis of purified LV-2A2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LV-2A2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

30,116 Da[Similar Products]

cysteine-rich secretory protein 3

cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; specific granule protein (28 kDa); specific granule protein of 28 kDa

CRISP-3; SGP28  [Similar Products]

CRIS3_HUMAN

CRISP3: Belongs to the CRISP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; specific granule; extracellular region

Biological Process: innate immune response; defense response

Krätzschmar J, Haendler B, Eberspaecher U, Roosterman D, Donner P, Schleuning WD (1996). The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 Eur J Biochem 15;236(3):827-36
Udby L, Bjartell A, Malm J, Egesten A, Lundwall A, Cowland JB, Borregaard N, Kjeldsen L (2005). Characterization and Localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the Human Male Reproductive Tract. J Androl ;26(3):333-42
Kosari F, Asmann YW, Cheville JC and Vasmatzis G (2002). Cysteine-rich Secretory Protein- 3: A Potential Biomarker for Prostate Cancer. Cancer Epidemiology Biomarkers & Prevention 11;1419-1426

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